Differentiated sap (4–6) gene expression of Candida albicans isolates from HIV-positive patients with oral candidiasis and commensals in healthy individuals

Meylani, Vita and Sembiring, Langkah and Fudholi, Ahmad and Wibawa, Tri Differentiated sap (4–6) gene expression of Candida albicans isolates from HIV-positive patients with oral candidiasis and commensals in healthy individuals. Microbial Pathogenesis.

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Abstract

Gene expression of SAP 4–6 based on the detection of mRNA was observed in Candida albicans isolates from HIVpositive patients with oral candidiasis and commensal from healthy individuals. The species of C. albicans strains were selectively isolated from both sources using CHROMagar Chromogenic Media. The obtained isolates were then induced to express SAP 4–6 using SAP 4–6 gene inducer media. Analysis of gene expression was performed on a molecular basis using the RT-PCR method. Molecular analysis of gene expression showed that the isolates CH3 from HIV-positive patients with oral candidiasis could express SAP 4–6 gene, while commensal isolates from healthy people could not. Based on the results of this study, it could be concluded that, in terms of molecular detection, only isolates from HIV-positive patients (CH3) could express their SAP 4–6 gene.

Item Type: Article
Subjects: Q Science > Q Science (General)
Divisions: Fakultas Keguruan dan Ilmu Pendidikan > Pendidikan Biologi > Artikel Dosen Pendidikan Biologi
Depositing User: Mrs Linda Amelia Oktavia
Date Deposited: 30 Jan 2023 04:59
Last Modified: 28 May 2023 14:37
URI: http://repositori.unsil.ac.id/id/eprint/8220

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